Basics of the high performance liquid chromatography

The monograph describes the basics of the high performance liquid chromatography (HPLC). The book was based mainly on lectures at the Siedlce University of Natural Sciences and Humanities. It discusses the fundamentals of chromatography including its history (containing chromatographs produced in Poland), the mechanism of the process (explaining why different substances move at different speeds along a chromatographic column or plate) and the division of chromato-graphic techniques. Then, the mechanism of the process was discussed based on the theory of chromatographic plate, mass balance equation in flow systems and empirical description (van Deemter equation). The next chapter discusses the basic chromatographic parameters and relationships between them, and derivation of the Purnell equation. Finally, the construction of the chromatographic apparatus is briefly discussed.